The Human HBU (Hemoglobin μ) CLIA Kit is specifically designed for the quantitative detection of HBU levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.HBU, also known as Hemoglobin μ, is a variant of hemoglobin found in human blood. It is essential for oxygen transport and plays a crucial role in maintaining the body's overall health. Abnormal levels of HBU have been linked to various hematological disorders, making it a valuable biomarker for diagnostic and research purposes.
With the Human HBU CLIA Kit, researchers can easily measure HBU levels in biological samples, enabling them to study the hemoglobin variant's role in health and disease. This kit provides a convenient and efficient solution for investigating HBU levels and their implications in various physiological and pathological conditions.
Product Name:
Human HBu (Hemoglobin, Mu) CLIA Kit
SKU:
HUES00537
Target:
Human HBu (Hemoglobin, Mu)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HBμ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HBμ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HBμ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HBμ. You can calculate the concentration of Human HBμ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
86-102
101-120
Average (%)
91
93
109
1:4
Range (%)
93-107
97-113
91-103
Average (%)
98
105
96
1:8
Range (%)
85-99
102-116
96-112
Average (%)
92
110
104
1:16
Range (%)
96-114
89-104
91-104
Average (%)
104
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.21
6.11
16.44
2.36
6.34
17.83
Standard deviation
0.26
0.61
1.28
0.3
0.55
1.48
C V (%)
11.76
9.98
7.79
12.71
8.68
8.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HBμ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HBμ in samples. No significant cross-reactivity or interference between Human HBμ and analogues was observed.
