The Human Heat Shock Protein Beta 2 (HSPB2) ELISA Kit is a reliable and accurate tool for the detection of HSPB2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for various research applications.HSPB2, also known as Heat Shock Protein 27 (HSP27), is a key player in cellular stress response and chaperone activity. It is involved in protecting cells from damage and regulating cellular functions, making it a crucial protein in various physiological and pathological processes.
This ELISA kit is essential for studying HSPB2's role in conditions such as neurodegenerative disorders, cardiovascular diseases, and cancer. By accurately measuring HSPB2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human HSPB2 ELISA Kit from Assay Genie is a valuable tool for researchers looking to explore the functions and implications of HSPB2 in health and disease.
Product Name:
Human Heat Shock Protein Beta 2 (HSPb2) ELISA Kit
Product Code:
HUDL01391
Size:
96 Assays
Target:
HSPb2
Synonyms:
HSP-B2, MKBP, HSP27, Heat Shock 27kDa Protein 2, DMPK-binding protein
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
