The Human Heat Shock Protein Beta-3 (HSPB3) ELISA Kit is specifically designed for the accurate measurement of HSPB3 levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and reproducible results for various research applications.HSPB3 is a key protein in the heat shock response pathway, playing a vital role in protecting cells from stress-induced damage and maintaining cellular homeostasis. Abnormalities in HSPB3 expression have been linked to various diseases, including neurodegenerative disorders, cancer, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and reliable performance, the Human Heat Shock Protein Beta-3 (HSPB3) ELISA Kit is an essential tool for researchers looking to explore the role of HSPB3 in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human Heat Shock Protein Beta 3 (HSPb3) ELISA Kit
Product Code:
HUDL01392
Size:
96 Assays
Target:
HSPb3
Synonyms:
HSP-B3, HSPL27, HSP27, HSP 17, Heat Shock 27kDa Protein 3, Heat shock 17 kDa protein
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
