Human Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ELISA Kit
The Human Heparin-Binding Epidermal Growth Factor-Like Growth Factor (HB-EGF) ELISA Kit is a powerful tool for accurately measuring HB-EGF levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results for a variety of research applications.HB-EGF is a key growth factor involved in various cellular processes, including cell proliferation, differentiation, and migration. It plays a crucial role in tissue repair, wound healing, and development, making it an important target for studying diseases such as cancer, cardiovascular disorders, and inflammatory conditions.
By using the Human HB-EGF ELISA Kit, researchers can gain valuable insights into the role of HB-EGF in physiological and pathological processes, leading to a better understanding of disease mechanisms and potential therapeutic interventions. Don't miss out on the opportunity to enhance your research with this cutting-edge ELISA kit.
Product Name:
Human Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
