The Human HEPc (Hepcidin) CLIA Kit is specifically designed for the precise measurement of hepcidin levels in human samples such as serum, plasma, and cell culture supernatants. This CLIA kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research endeavors.Hepcidin is a key regulator of iron homeostasis, playing a crucial role in controlling iron absorption and distribution in the body.
Dysregulation of hepcidin levels has been linked to various disorders such as anemia, hemochromatosis, and inflammation-related diseases. Therefore, this kit serves as a vital resource for investigating the role of hepcidin in these conditions and exploring potential therapeutic interventions.
Product Name:
Human Hepc (Hepcidin) CLIA Kit
SKU:
HUES00075
Target:
Human Hepc (Hepcidin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Hepc. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Hepc. You can calculate the concentration of Human Hepc in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
86-100
99-112
Average (%)
108
91
105
1:4
Range (%)
95-110
99-115
100-116
Average (%)
101
106
108
1:8
Range (%)
86-100
100-114
96-110
Average (%)
91
108
101
1:16
Range (%)
87-99
91-104
96-110
Average (%)
93
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.27
211.26
844.52
89.92
190.73
828.61
Standard deviation
10.54
20.05
66.38
9.25
14.13
80.71
C V (%)
11.42
9.49
7.86
10.29
7.41
9.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Hepc concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Hepc in samples. No significant cross-reactivity or interference between Human Hepc and analogues was observed.
