Description
Human Hepc (Hepcidin) ELISA Kit
The Hepcidin (Hepc) ELISA Kit is a specialized assay designed for the precise quantitative detection of Hepcidin levels in human biological samples. Hepcidin, a key regulator of iron metabolism, plays a crucial role in modulating iron homeostasis by regulating iron absorption and distribution within the body.
Accurate measurement of Hepcidin levels is essential for understanding its implications in iron-related disorders, such as anemia and iron overload conditions. This ELISA kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results. Manufactured under strict quality control measures, the Hepcidin ELISA Kit provides reliable performance and user-friendly operation, making it an optimal choice for both research and clinical applications. Rely on the Hepcidin ELISA Kit from Assay Genie for precise and dependable quantification of this pivotal biomarker in your studies.
| Product Name: | Human Hepc (Hepcidin) ELISA Kit |
| Product Code: | AEES00050 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 62.50-4000 pg/mL |
| Sensitivity: | 37.50 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human Hepc in samples. No significant cross-reactivity or interference between Human Hepc and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human Hepc concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Hepc. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Hepc. You can calculate the concentration of Human Hepc in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
