Human Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) ELISA Kit
The Human Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) ELISA Kit is a reliable tool for quantitative detection of HNRPA2B1 levels in human samples including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures accurate and reproducible results for various research applications.HNRPA2B1 is a key protein involved in RNA processing and gene regulation, playing a critical role in cellular functions such as RNA splicing and stability.
Dysregulation of HNRPA2B1 has been linked to various diseases including cancer, autoimmune disorders, and neurological conditions, making it a valuable biomarker for studying disease mechanisms and developing targeted therapies.Overall, the Human HNRPA2B1 ELISA Kit offers researchers a powerful tool to investigate the role of this essential protein in health and disease, paving the way for advancements in biomedical research and clinical diagnostics.
Product Name:
Human Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) ELISA Kit
Product Code:
HUDL01348
Size:
96 Assays
Target:
HNRPA2B1
Synonyms:
hnRNP-A2/B1, hnRNPA2B1, RA33, HnRNPA2/B1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
