Human HHIP (Hedgehog Interacting Protein) ELISA Kit
The Human HHIP (Hedgehog Interacting Protein) ELISA Kit is specifically designed for the accurate detection of HHIP levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and consistent results, making it suitable for a variety of research purposes.HHIP is a key protein that interacts with the hedgehog signaling pathway, which plays a critical role in various cellular processes such as development, tissue regeneration, and cancer progression.
Understanding the levels of HHIP can provide valuable insights into the regulation of this pathway and its implications in diseases such as cancer, developmental disorders, and wound healing.Overall, the Human HHIP ELISA Kit is a valuable tool for researchers studying hedgehog signaling and its related pathways, providing reliable data for further investigation and potential therapeutic developments.
Product Name:
Human HHIP (Hedgehog Interacting Protein) ELISA Kit
SKU:
HUES02016
Target:
Human HHIP (Hedgehog Interacting Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HHIP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HHIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HHIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HHIP. You can calculate the concentration of Human HHIP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
95-108
91-106
Average (%)
91
101
96
1:4
Range (%)
94-106
83-93
85-95
Average (%)
100
88
90
1:8
Range (%)
92-106
84-94
82-95
Average (%)
98
89
87
1:16
Range (%)
86-102
79-90
80-92
Average (%)
93
85
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
3.08
7.5
1.05
3.06
6.77
Standard deviation
0.05
0.17
0.38
0.05
0.15
0.29
C V (%)
4.85
5.52
5.07
4.76
4.9
4.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HHIP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HHIP in samples. No significant cross-reactivity or interference between Human HHIP and analogues was observed.
