Human High Mobility Group Protein 1 (HMG1) ELISA Kit
The Human High Mobility Group Protein 1 (HMG1) ELISA Kit is specifically designed for the accurate and reliable detection of HMG1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures consistent and reproducible results, making it an invaluable tool for a variety of research applications.HMG1 is a key protein involved in DNA binding and gene regulation, playing a crucial role in various cellular processes such as inflammation, immune response, and cancer progression. Its dysregulation has been linked to numerous diseases, including autoimmune disorders, sepsis, and cancer, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
The Human HMG1 ELISA Kit offers researchers a comprehensive solution for studying the role of HMG1 in disease pathogenesis and treatment, providing accurate and precise measurement of HMG1 levels in biological samples. With its ease of use and reliable performance, this kit is a valuable asset for advancing scientific knowledge and developing new strategies for disease management.
Product Name:
Human High Mobility Group Protein 1 (HMG1) ELISA Kit
Product Code:
HUDL01340
Size:
96 Assays
Target:
HMG1
Synonyms:
HMGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
