The Human Histone Deacetylase 5 (HDAC5) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of HDAC5 levels in human biological samples. This kit is ideal for researchers studying epigenetic regulation and gene expression, as HDAC5 is a key player in chromatin remodeling and gene transcription.HDAC5 is a histone deacetylase enzyme that plays a critical role in various cellular processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of HDAC5 has been linked to a wide range of diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, measuring HDAC5 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and reproducibility, the Human HDAC5 ELISA Kit is a valuable tool for researchers looking to study the role of HDAC5 in health and disease. The kit is easy to use and delivers accurate results, making it a reliable choice for a wide range of research applications.
Product Name:
Human Histone Deacetylase 5 (HDAC5) ELISA Kit
Product Code:
HUDL01318
Size:
96 Assays
Target:
HDAC5
Synonyms:
HD5, NY-CO-9
Detection Method:
Competition
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
