The Human HLA-A (Leukocyte Antigen A) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of HLA-A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional specificity, ensuring precise and reproducible results for a variety of research applications.HLA-A is a crucial component of the human immune system, playing a vital role in immune response and identifying foreign pathogens.
Abnormalities in HLA-A expression have been linked to various autoimmune diseases, infections, and transplant rejection, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human HLA-A CLIA Kit provides researchers with a powerful tool for investigating the role of HLA-A in health and disease, offering unparalleled accuracy and sensitivity for their research needs.
Product Name:
Human HLA-A (Leukocyte Antigen A) CLIA Kit
SKU:
HUES00934
Target:
Human HLA-A (Leukocyte Antigen A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HLA-A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HLA-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HLA-A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HLA-A. You can calculate the concentration of Human HLA-A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
86-98
98-115
Average (%)
101
92
105
1:4
Range (%)
93-104
92-106
101-116
Average (%)
99
97
109
1:8
Range (%)
93-107
100-117
99-113
Average (%)
98
108
106
1:16
Range (%)
90-102
97-111
87-102
Average (%)
95
104
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.01
435.08
1940.99
179.6
444.34
1834.06
Standard deviation
19.93
43.16
131.6
16.06
31.19
137.74
C V (%)
10.77
9.92
6.78
8.94
7.02
7.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HLA-A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HLA-A in samples. No significant cross-reactivity or interference between Human HLA-A and analogues was observed.
