The Human HLA-B27 (Human Leukocyte Antigen B27) ELISA Kit is a valuable tool for the precise quantification of HLA-B27 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.HLA-B27 is a critical component of the immune system, playing a key role in antigen presentation and immune response.
Its association with autoimmune diseases such as ankylosing spondylitis and reactive arthritis makes it a significant biomarker for understanding and investigating these conditions. The Human HLA-B27 ELISA Kit facilitates the study of HLA-B27 levels in diverse physiological and pathological contexts, contributing to the development of potential diagnostic and therapeutic strategies in autoimmune diseases.
Product Name:
Human HLA-B27 ELISA Kit
SKU:
HUES01429
Target:
Human HLA-B27 (Human Leukocyte Antigen B27)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HLA-B27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HLA-B27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HLA-B27. You can calculate the concentration of Human HLA-B27 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
87-101
98-114
Average (%)
96
95
104
1:4
Range (%)
89-99
87-99
84-98
Average (%)
94
92
90
1:8
Range (%)
87-102
81-94
83-98
Average (%)
94
87
89
1:16
Range (%)
88-102
80-91
85-99
Average (%)
94
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
97
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
1.86
8.56
0.97
1.91
8.52
Standard deviation
0.06
0.09
0.44
0.05
0.1
0.36
C V (%)
6.06
4.84
5.14
5.15
5.24
4.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HLA-B27 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HLA-B27 in samples. No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed.
