The Human HRG (Histidine-rich glycoprotein) CLIA Kit is a cutting-edge tool for measuring HRG levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research purposes.HRG is a multifunctional protein that plays a critical role in numerous biological processes, including blood coagulation, angiogenesis, and immune response modulation. Dysregulation of HRG has been linked to various diseases, making it a valuable biomarker for investigating conditions such as cancer, inflammatory disorders, and thrombosis.
With its advanced technology and proven performance, the Human HRG CLIA Kit is an indispensable resource for researchers seeking to unravel the complexities of HRG biology and its implications for human health. Get your hands on this innovative kit today and take your research to the next level.
Product Name:
Human HRG (Histidine Rich Glycoprotein) CLIA Kit
SKU:
HUES00302
Target:
Human HRG (Histidine Rich Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HRG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HRG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HRG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HRG. You can calculate the concentration of Human HRG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
87-100
89-99
Average (%)
101
94
94
1:4
Range (%)
98-112
93-109
101-114
Average (%)
104
99
108
1:8
Range (%)
98-112
104-118
103-119
Average (%)
103
110
109
1:16
Range (%)
92-108
98-113
92-103
Average (%)
98
106
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
98-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.22
281.65
802.54
94.66
296.7
782.41
Standard deviation
10.75
28.53
65.97
10.79
24.39
56.88
C V (%)
11.78
10.13
8.22
11.4
8.22
7.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HRG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HRG in samples. No significant cross-reactivity or interference between Human HRG and analogues was observed.
