Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit
The Human HSP-27 (HSPB1) Heat Shock Protein 27 ELISA Kit is specifically designed for the precise measurement of HSP-27 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.HSP-27, also known as Heat Shock Protein 27, is a key protein involved in cellular stress response and protection against various environmental stresses. Its role in regulating cell survival and apoptosis makes it a valuable biomarker for studying conditions such as cancer, neurodegenerative disorders, and cardiovascular diseases.
The reliable detection of HSP-27 levels using this ELISA kit can provide crucial insights into disease mechanisms and potential therapeutic interventions.Overall, the Human HSP-27 Heat Shock Protein 27 ELISA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of HSP-27 in health and disease, offering accurate and reliable measurements to support their research endeavors.
Product Name:
Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit
SKU:
HUES02812
Target:
Human HSP-27/HSPB1 (Heat Shock Protein 27)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-27/HSPB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-27/HSPB1. You can calculate the concentration of Human HSP-27/HSPB1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
97-112
86-100
Average (%)
99
103
92
1:4
Range (%)
95-109
81-93
94-110
Average (%)
102
86
101
1:8
Range (%)
94-107
81-93
93-106
Average (%)
101
87
100
1:16
Range (%)
97-108
81-94
95-108
Average (%)
103
86
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
102
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
83-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.5
6.9
21.0
2.6
7.2
22.3
Standard deviation
0.2
0.3
1.0
0.2
0.3
1.0
C V (%)
8
4.35
4.76
7.69
4.17
4.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HSP-27/HSPB1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-27/HSPB1 in samples. No significant cross-reactivity or interference between Human HSP-27/HSPB1 and analogues was observed.
