Human HSP-60/HSPD1 (Heat Shock Protein 60) CLIA Kit
The Human HSP-60 (Hspd1) Heat Shock Protein 60 CLIA Kit is specifically designed for the accurate quantification of HSP-60 levels in human serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.HSP-60, also known as Heat Shock Protein 60, is a key molecular chaperone involved in cellular stress response and maintaining protein homeostasis. It plays a vital role in regulating protein folding, transport, and degradation, making it a crucial factor in various physiological and pathological processes.
Elevated levels of HSP-60 have been linked to inflammatory conditions, autoimmune diseases, and cancer, underscoring its importance as a potential biomarker for disease diagnosis and prognosis. By using this CLIA kit, researchers can accurately measure HSP-60 levels, furthering our understanding of its biological functions and potential therapeutic applications.
Product Name:
Human HSP-60/HSPD1 (Heat Shock Protein 60) CLIA Kit
SKU:
HUES01029
Target:
Human HSP-60/HSPD1 (Heat Shock Protein 60)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-60/HSPD1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-60/HSPD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-60/HSPD1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSP-60/HSPD1. You can calculate the concentration of Human HSP-60/HSPD1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
87-98
93-107
Average (%)
91
92
100
1:4
Range (%)
103-119
97-109
93-107
Average (%)
110
103
100
1:8
Range (%)
84-100
99-116
99-114
Average (%)
91
107
104
1:16
Range (%)
86-97
102-120
100-117
Average (%)
92
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
95-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
2.6
6.78
1.11
2.8
6.83
Standard deviation
0.12
0.29
0.44
0.11
0.33
0.52
C V (%)
11.43
11.15
6.49
9.91
11.79
7.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSP-60/HSPD1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-60/HSPD1 in samples. No significant cross-reactivity or interference between Human HSP-60/HSPD1 and analogues was observed.
