Human HSP-60/HSPD1 (Heat Shock Protein 60) ELISA Kit
The Human HSP-60 (HSPD1) Heat Shock Protein 60 ELISA Kit is a powerful tool for accurately measuring levels of HSP-60 in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing researchers with dependable and consistent results for various research purposes.HSP-60, also known as HSPD1, is a critical chaperone protein involved in cellular stress response and maintaining protein structure. Its role in various diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions makes it a valuable biomarker for understanding disease progression and developing potential therapeutic interventions.
By using the Human HSP-60 (HSPD1) Heat Shock Protein 60 ELISA Kit, researchers can gain valuable insights into the role of HSP-60 in different biological processes and disease pathways, ultimately advancing our understanding of human health and potential treatment strategies.
Product Name:
Human HSP-60/HSPD1 (Heat Shock Protein 60) ELISA Kit
SKU:
HUES02814
Target:
Human HSP-60/HSPD1 (Heat Shock Protein 60)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-60/HSPD1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-60/HSPD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-60/HSPD1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-60/HSPD1. You can calculate the concentration of Human HSP-60/HSPD1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
96-114
87-100
Average (%)
103
104
93
1:4
Range (%)
95-108
87-101
92-109
Average (%)
101
93
100
1:8
Range (%)
94-109
82-95
91-105
Average (%)
101
87
97
1:16
Range (%)
96-108
88-99
97-110
Average (%)
101
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.7
12.9
36.9
4.8
12.0
34.6
Standard deviation
0.3
0.8
1.8
0.3
0.7
1.8
C V (%)
6.38
6.2
4.88
6.25
5.83
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HSP-60/HSPD1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-60/HSPD1 in samples. No significant cross-reactivity or interference between Human HSP-60/HSPD1 and analogues was observed.
