Human HSPG (Heparan Sulfate Proteoglycan) CLIA Kit
The Human HSPG (Heparan Sulfate Proteoglycan) CLIA Kit is a cutting-edge tool designed for the precise quantification of HSPG levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring robust and consistent results that are perfect for various research applications.HSPG is a key component of the extracellular matrix and is essential for cell signaling, growth, and adhesion. Dysregulation of HSPG has been implicated in various diseases, including cancer, inflammation, and neurodegenerative disorders, making it a valuable biomarker for studying and potentially targeting these conditions.
With the Human HSPG CLIA Kit, researchers can accurately measure HSPG levels in serum, plasma, and tissue culture supernatants, providing valuable insights into the role of HSPG in health and disease. This easy-to-use kit is a must-have for any lab conducting research on HSPG and its associated pathways.
Product Name:
Human HSPG (Heparan Sulfate Proteoglycan) CLIA Kit
SKU:
HUES00303
Target:
Human HSPG (Heparan Sulfate Proteoglycan)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSPG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSPG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSPG. You can calculate the concentration of Human HSPG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
99-114
90-103
Average (%)
94
107
97
1:4
Range (%)
101-115
85-95
91-105
Average (%)
107
90
96
1:8
Range (%)
92-106
91-104
98-115
Average (%)
98
99
105
1:16
Range (%)
97-111
88-102
96-109
Average (%)
103
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
84-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
201.22
361.48
1532.83
194.74
332.89
1559.48
Standard deviation
19.82
31.63
155.12
17.96
28.96
134.27
C V (%)
9.85
8.75
10.12
9.22
8.7
8.61
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSPG concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human HSPG in samples. No significant cross-reactivity or interference between Human HSPG and analogues was observed.
