The Human HTRA1 (High Temperature Requirement A Serine Peptidase 1) CLIA Kit is designed for the accurate detection of HTRA1 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.HTRA1 is a serine protease enzyme that plays a crucial role in various physiological processes, including cell differentiation, tissue remodeling, and apoptosis.
Dysregulation of HTRA1 has been linked to various diseases, such as cancer, Alzheimer's disease, and osteoarthritis, making it an essential biomarker for studying these conditions and developing potential therapies.This CLIA kit offers a simple and efficient way to measure HTRA1 levels in human samples, providing valuable insights into disease progression and potential treatment options. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Human HTRA1 (HtrA Serine Peptidase 1) CLIA Kit
SKU:
HUES00305
Target:
Human HTRA1 (HtrA Serine Peptidase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HTRA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HTRA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HTRA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HTRA1. You can calculate the concentration of Human HTRA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-97
95-105
102-120
Average (%)
90
100
110
1:4
Range (%)
85-97
87-101
96-111
Average (%)
92
93
102
1:8
Range (%)
96-109
95-108
87-98
Average (%)
102
101
93
1:16
Range (%)
103-117
98-116
87-100
Average (%)
109
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
250.96
619.38
1810.37
244.59
672.66
1936.43
Standard deviation
25.37
57.91
141.57
28.79
63.63
145.43
C V (%)
10.11
9.35
7.82
11.77
9.46
7.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HTRA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HTRA1 in samples. No significant cross-reactivity or interference between Human HTRA1 and analogues was observed.
