Human Hydrogen Voltage Gated Channel Protein 1 (HVCN1) ELISA Kit
The Human Hydrogen Voltage-Gated Channel Protein 1 (HVCN1) ELISA Kit is specifically designed for the accurate and precise detection of HVCN1 levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.HVCN1 is a critical protein involved in regulating hydrogen ion movement across cell membranes, impacting various cellular functions and processes.
Dysregulation of HVCN1 has been linked to various diseases and disorders, including inflammation, immune response, and neurological disorders. As such, studying HVCN1 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human HVCN1 ELISA Kit offers researchers a powerful tool for studying the role of HVCN1 in health and disease, providing accurate and reliable data for further scientific investigations.
Product Name:
Human Hydrogen Voltage Gated Channel Protein 1 (HVCN1) ELISA Kit
Product Code:
HUDL01412
Size:
96 Assays
Target:
HVCN1
Synonyms:
Hv1, VSOP, Voltage Sensor Domain-Only Protein, Hydrogen voltage-gated channel 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
