The Human IAPP (Islet Amyloid Polypeptide) ELISA Kit is specifically designed for the precise measurement of IAPP levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, researchers can rely on this kit to deliver consistent and accurate results for a variety of research applications.IAPP, also known as amylin, is a peptide hormone that plays a key role in glucose metabolism and regulation of insulin secretion.
Dysregulation of IAPP has been linked to conditions such as diabetes and Alzheimer's disease, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.By using the Human IAPP ELISA Kit, researchers can gain valuable insights into the role of IAPP in disease pathology and development, ultimately contributing to the advancement of biomedical research and clinical diagnostics.
Product Name:
Human IAPP (Islet Amyloid Polypeptide) ELISA Kit
SKU:
HUES01544
Target:
Human IAPP (Islet Amyloid Polypeptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IAPP. During the reaction, IAPP in the sample or standard competes with a fixed amount of IAPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IAPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IAPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
97-112
94-108
Average (%)
104
105
102
1:4
Range (%)
85-98
91-102
98-113
Average (%)
92
96
106
1:8
Range (%)
88-102
93-105
95-110
Average (%)
94
99
102
1:16
Range (%)
91-103
88-100
99-115
Average (%)
96
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.9
485.9
1754.1
197.0
478.4
1764.5
Standard deviation
9.9
21.9
84.2
12.6
21.0
84.7
C V (%)
5.11
4.51
4.8
6.4
4.39
4.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of IAPP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes IAPP in samples. No significant cross-reactivity or interference between IAPP and analogues was observed.
