The Human IgA2 (Immunoglobulin A2) CLIA Kit is specifically designed for the accurate and precise detection of IgA2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Immunoglobulin A2 is an important antibody that plays a key role in the immune response, particularly at mucosal surfaces. It is involved in defending the body against infections and other foreign substances, making it a valuable biomarker for studying immune function and potential immunodeficiency disorders.
With its advanced technology and optimized protocols, the Human IgA2 CLIA kit from Assay Genie provides researchers with a powerful tool for investigating IgA2 levels in various biological samples, paving the way for new discoveries in immunology and disease research.
Product Name:
Human IgA2 (Immumoglobulin A2) CLIA Kit
SKU:
HUES00819
Target:
Human IgA2 (Immumoglobulin A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgA2. You can calculate the concentration of Human IgA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
85-97
87-98
Average (%)
99
92
92
1:4
Range (%)
103-118
88-104
100-116
Average (%)
110
95
107
1:8
Range (%)
86-98
97-110
94-107
Average (%)
93
103
99
1:16
Range (%)
101-117
100-114
96-109
Average (%)
107
108
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
90-106
96
Cell culture media (n=5)
84-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.1
14.33
39.85
4.91
13.3
37.38
Standard deviation
0.53
1.36
3.24
0.58
1.17
2.96
C V (%)
10.39
9.49
8.13
11.81
8.8
7.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgA2 in samples. No significant cross-reactivity or interference between Human IgA2 and analogues was observed.
