Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) CLIA Kit
The Human IGF2BP3 (Insulin-like growth factor 2 mRNA-binding protein 3) CLIA Kit is specifically designed for the precise measurement of IGF2BP3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IGF2BP3 is a key protein involved in binding to mRNA, regulating gene expression, and promoting cell growth and proliferation.
Dysregulation of IGF2BP3 has been linked to various diseases including cancer, diabetes, and developmental disorders, making it a crucial biomarker for studying these conditions and potential therapeutic interventions.Don't miss the opportunity to study IGF2BP3 with precision and reliability using the Human IGF2BP3 CLIA Kit. Order yours today and advance your research in the field of molecular biology and disease pathology.
Product Name:
Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) CLIA Kit
SKU:
HUES00315
Target:
Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGF2BP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF2BP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF2BP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGF2BP3. You can calculate the concentration of Human IGF2BP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
98-115
91-102
Average (%)
108
105
97
1:4
Range (%)
102-120
96-109
91-105
Average (%)
109
103
96
1:8
Range (%)
96-110
92-106
98-112
Average (%)
103
100
106
1:16
Range (%)
94-110
85-98
95-107
Average (%)
101
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
94-111
102
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.69
552.79
1734.35
191.17
540.37
1735.17
Standard deviation
17.39
42.01
187.31
20.95
64.79
135.69
C V (%)
9.52
7.6
10.8
10.96
11.99
7.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGF2BP3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF2BP3 in samples. No significant cross-reactivity or interference between Human IGF2BP3 and analogues was observed.
