Human IGF2R (Insulin Like Growth Factor 2 Receptor) ELISA Kit
The Human IGF2R (Insulin-Like Growth Factor 2 Receptor) ELISA Kit is a specialized tool for the accurate measurement of IGF2R levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and reproducible results, making it an excellent choice for a variety of research purposes.The IGF2R protein is critical for regulating the activity of insulin-like growth factor 2 (IGF2) and plays a key role in cell growth, development, and metabolism.
Dysregulation of IGF2R has been linked to various diseases, including cancer, diabetes, and developmental disorders, highlighting its importance as a potential biomarker for disease detection and therapeutic development.By using the Human IGF2R ELISA Kit, researchers can gain valuable insights into the role of IGF2R in disease progression and explore potential treatment options. This kit is a valuable tool for studying the molecular mechanisms underlying IGF2R-related conditions and accelerating biomedical research efforts.
Product Name:
Human IGF2R (Insulin Like Growth Factor 2 Receptor) ELISA Kit
SKU:
HUES01652
Target:
Human IGF2R (Insulin Like Growth Factor 2 Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF2R. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF2R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF2R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF2R. You can calculate the concentration of Human IGF2R in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
94-107
94-109
Average (%)
105
99
99
1:4
Range (%)
91-103
83-98
88-101
Average (%)
96
90
93
1:8
Range (%)
93-110
86-98
86-99
Average (%)
100
91
93
1:16
Range (%)
89-104
84-96
87-103
Average (%)
97
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
101
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.06
3.37
17.85
1.96
3.39
18.61
Standard deviation
0.11
0.17
0.79
0.12
0.19
0.58
C V (%)
5.34
5.04
4.43
6.12
5.6
3.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGF2R concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF2R in samples. No significant cross-reactivity or interference between Human IGF2R and analogues was observed.
