Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) CLIA Kit
The Human IGFBP-1 (Insulin-like Growth Factor Binding Protein-1) CLIA Kit is a highly sensitive and specific assay designed for the precise detection of IGFBP-1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an invaluable tool for a variety of research applications.IGFBP-1 is a key protein involved in regulating the activity of insulin-like growth factors, impacting cell growth, differentiation, and survival. Abnormal levels of IGFBP-1 have been linked to various health conditions, including diabetes, metabolic disorders, and certain cancers, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
The Human IGFBP-1 CLIA Kit from Assay Genie offers researchers a high-quality solution for accurately measuring IGFBP-1 levels, facilitating in-depth investigations into the role of this protein in health and disease. Trust in the precision and performance of this kit to advance your research and uncover new insights into the complex mechanisms of IGFBP-1 biology.
Product Name:
Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) CLIA Kit
SKU:
HUES00319
Target:
Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFBP-1. You can calculate the concentration of Human IGFBP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
100-114
92-102
Average (%)
96
106
97
1:4
Range (%)
102-116
99-114
86-99
Average (%)
110
106
93
1:8
Range (%)
101-118
86-99
96-109
Average (%)
108
93
102
1:16
Range (%)
88-100
84-97
99-115
Average (%)
93
90
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
97
EDTA plasma (n=5)
92-106
97
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.38
194.91
799.38
104.45
186.91
801.01
Standard deviation
8.31
21.48
87.13
9.67
15.14
84.35
C V (%)
8.28
11.02
10.9
9.26
8.1
10.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFBP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-1 in samples. No significant cross-reactivity or interference between Human IGFBP-1 and analogues was observed.
