Human IGFBP-2 (Insulin Like Growth Factor Binding Protein 2) CLIA Kit
The Human IGFBP-2 (Insulin-like Growth Factor Binding Protein 2) CLIA Kit is a reliable tool for the quantitative measurement of IGFBP-2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for various research applications.IGFBP-2 is a key protein that binds to insulin-like growth factors, regulating their activity and influencing cell growth and development.
Changes in IGFBP-2 levels have been associated with conditions like cancer, diabetes, and neurodegenerative diseases, highlighting its significance as a potential biomarker for studying these disorders and developing therapeutic strategies.Overall, the Human IGFBP-2 CLIA Kit provides researchers with a valuable tool to investigate the role of IGFBP-2 in various physiological and pathological processes, paving the way for new insights and advancements in biomedical research.
Product Name:
Human IGFBP-2 (Insulin Like Growth Factor Binding Protein 2) CLIA Kit
SKU:
HUES00322
Target:
Human IGFBP-2 (Insulin Like Growth Factor Binding Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFBP-2. You can calculate the concentration of Human IGFBP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
100-114
100-119
Average (%)
94
105
109
1:4
Range (%)
100-117
91-108
103-116
Average (%)
108
99
110
1:8
Range (%)
91-102
102-116
100-118
Average (%)
97
108
109
1:16
Range (%)
92-107
102-117
95-106
Average (%)
99
109
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-114
107
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.13
412.64
1492.96
220.83
445.97
1425.37
Standard deviation
26.94
30.82
157.21
24.34
44.51
98.35
C V (%)
12.76
7.47
10.53
11.02
9.98
6.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFBP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-2 in samples. No significant cross-reactivity or interference between Human IGFBP-2 and analogues was observed.
