Description
Human IGFBP-2 (Insulin-like Growth Factor Binding Protein 2) ELISA Kit
The Human IGFBP-2 (Insulin-like Growth Factor Binding Protein 2) ELISA Kit is meticulously crafted for the quantitative detection of IGFBP-2 levels in various human biological samples. IGFBP-2, a critical protein, is a binding protein that modulates the actions of insulin-like growth factors. It plays a pivotal role in regulating cell growth, proliferation, and differentiation processes. Accurate measurement of IGFBP-2 levels is fundamental in understanding its intricate involvement in cellular growth regulation and developmental processes.
This ELISA kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control measures, this kit guarantees reliable performance and user-friendly operation, making it an ideal choice for both research and clinical applications. For accurate and dependable quantification of IGFBP-2, trust Assay Genie's IGFBP-2 ELISA Kit for your research needs.
| Product Name: | Human IGFBP-2 (Insulin-like Growth Factor Binding Protein 2) ELISA Kit |
| Product Code: | AEES00074 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 1.56-100 ng/mL |
| Sensitivity: | 0.94 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human IGFBP-2 in samples. No significant cross-reactivity or interference between Human IGFBP-2 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human IGFBP-2 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGFBP-2. You can calculate the concentration of Human IGFBP-2 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
