Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) CLIA Kit
The Human IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) CLIA Kit is a powerful tool for the detection of IGFBP-4 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it a valuable asset for various research applications.IGFBP-4 is a key protein that interacts with insulin-like growth factors, regulating their bioavailability and activity. It plays a crucial role in cell growth, differentiation, and survival, making it a significant player in various physiological processes.
Dysregulation of IGFBP-4 has been linked to conditions such as cancer, diabetes, and metabolic disorders, highlighting its importance as a biomarker for studying these diseases and developing potential treatments.Overall, the Human IGFBP-4 CLIA Kit offers researchers a reliable and accurate method for measuring IGFBP-4 levels, providing insights that can advance our understanding of disease mechanisms and therapeutic strategies.
Product Name:
Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) CLIA Kit
SKU:
HUES00321
Target:
Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFBP-4. You can calculate the concentration of Human IGFBP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
91-105
98-111
Average (%)
95
98
105
1:4
Range (%)
91-105
98-110
97-111
Average (%)
97
104
105
1:8
Range (%)
89-103
99-112
103-120
Average (%)
96
105
110
1:16
Range (%)
90-102
98-109
89-105
Average (%)
96
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
93-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.99
3.97
18.71
1.95
4.25
18.01
Standard deviation
0.24
0.36
1.99
0.22
0.31
1.21
C V (%)
12.06
9.07
10.64
11.28
7.29
6.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFBP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-4 in samples. No significant cross-reactivity or interference between Human IGFBP-4 and analogues was observed.
