Human IGFBP-6 (Insulin Like Growth Factor Binding Protein 6) CLIA Kit
The Human IGFBP-6 (Insulin-like Growth Factor Binding Protein 6) CLIA Kit is a powerful tool for the quantitative measurement of IGFBP-6 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable asset for various research applications.IGFBP-6 is a key regulator of insulin-like growth factors, playing a crucial role in cell growth, differentiation, and survival.
Dysregulation of IGFBP-6 is associated with various health conditions, including cancer, metabolic disorders, and skeletal growth abnormalities, making it a promising target for therapeutic interventions and diagnostic purposes.By utilizing the Human IGFBP-6 CLIA Kit, researchers can gain deeper insights into the role of IGFBP-6 in disease pathogenesis and treatment strategies, ultimately advancing our understanding of complex biological processes and paving the way for innovative therapies.
Product Name:
Human IGFBP-6 (Insulin Like Growth Factor Binding Protein 6) CLIA Kit
SKU:
HUES00325
Target:
Human IGFBP-6 (Insulin Like Growth Factor Binding Protein 6)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFBP-6. You can calculate the concentration of Human IGFBP-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
86-97
100-114
Average (%)
101
91
108
1:4
Range (%)
92-105
97-111
84-99
Average (%)
99
103
91
1:8
Range (%)
91-105
104-121
91-105
Average (%)
96
110
97
1:16
Range (%)
99-113
98-115
101-115
Average (%)
105
106
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.99
175.36
824.92
112.23
179.85
807.28
Standard deviation
14.16
17.62
51.23
10.67
15.0
75.64
C V (%)
12.76
10.05
6.21
9.51
8.34
9.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFBP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-6 in samples. No significant cross-reactivity or interference between Human IGFBP-6 and analogues was observed.
