Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) CLIA Kit
The Human IGFBP-7 (Insulin-like Growth Factor Binding Protein 7) CLIA Kit is a powerful tool for detecting IGFBP-7 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research purposes.IGFBP-7 is a key protein involved in regulating the activity of insulin-like growth factors and has been implicated in various diseases, including cancer, cardiovascular diseases, and metabolic disorders.
By measuring IGFBP-7 levels, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Human IGFBP-7 CLIA Kit is essential for studying the role of IGFBP-7 in health and disease, providing valuable information for further research and the development of targeted treatments.
Product Name:
Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) CLIA Kit
SKU:
HUES00323
Target:
Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFBP-7. You can calculate the concentration of Human IGFBP-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
98-112
90-103
Average (%)
95
106
97
1:4
Range (%)
100-117
92-104
91-103
Average (%)
109
97
97
1:8
Range (%)
89-103
97-112
89-103
Average (%)
94
104
95
1:16
Range (%)
89-99
88-100
93-107
Average (%)
94
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.58
4.07
0.58
1.6
4.41
Standard deviation
0.07
0.19
0.38
0.07
0.15
0.34
C V (%)
12.73
12.03
9.34
12.07
9.38
7.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFBP-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed.
