The Human IgG (Immunoglobulin G) CLIA Kit is a cutting-edge diagnostic tool designed for the accurate quantification of IgG levels in human serum samples. This kit boasts high sensitivity and specificity, providing researchers and healthcare professionals with reliable and reproducible results for a variety of applications.Immunoglobulin G (IgG) is a key component of the human immune system, playing a critical role in the body's defense against infections and diseases. Abnormalities in IgG levels can indicate underlying health issues and autoimmune disorders, making it a valuable biomarker for diagnostic and research purposes.
Whether investigating immune responses, monitoring disease progression, or evaluating treatment efficacy, the Human IgG CLIA Kit offers a precise and efficient solution for measuring IgG levels in clinical samples. Trust in this innovative kit to support your research and diagnostic objectives with accuracy and confidence.
Product Name:
Human IgG (Immunoglobulin G) CLIA Kit
SKU:
HUES00146
Target:
Human IgG (Immunoglobulin G)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgG. You can calculate the concentration of Human IgG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
95-112
91-101
Average (%)
95
103
96
1:4
Range (%)
90-102
92-110
91-104
Average (%)
96
100
98
1:8
Range (%)
101-115
91-103
84-95
Average (%)
109
96
90
1:16
Range (%)
98-113
102-114
87-100
Average (%)
105
108
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
96-114
104
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.39
195.14
825.54
87.5
195.78
850.3
Standard deviation
11.52
16.92
77.93
10.7
14.84
88.77
C V (%)
12.47
8.67
9.44
12.23
7.58
10.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgG in samples. No significant cross-reactivity or interference between Human IgG and analogues was observed.
