The Human IL-10 (Interleukin-10) ELISA Kit is meticulously developed to facilitate the quantitative measurement of IL-10 levels in various biological samples. Interleukin-10, a crucial anti-inflammatory cytokine, plays a vital role in modulating the immune response by suppressing inflammation and promoting immune tolerance. IL-10 is involved in regulating various immune responses, inhibiting pro-inflammatory cytokines, and maintaining immune homeostasis. Accurate quantification of IL-10 levels is essential for understanding its immunomodulatory functions and its implications in inflammation-related disorders.
Our ELISA kit offers superior sensitivity and specificity, ensuring accurate and reproducible results crucial for investigating the immunoregulatory roles of IL-10 in different biological contexts. Manufactured under strict quality control measures, this kit delivers robust performance and user-friendly procedures, making it an ideal choice for cutting-edge research applications. Trust Assay Genie's IL-10 ELISA Kit for precise and dependable quantification of this key immunomodulatory biomarker in your studies.
Product Name:
Human IL-10 (Interleukin10)ELISA Kit
SKU:
AEES00162
Target:
Human IL-10 (Interleukin10)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-10. You can calculate the concentration of Human IL-10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
90-105
90-104
Average (%)
104
97
95
1:4
Range (%)
93-111
92-108
88-103
Average (%)
101
99
95
1:8
Range (%)
97-111
89-100
84-96
Average (%)
103
94
91
1:16
Range (%)
101-117
90-103
91-106
Average (%)
107
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
94
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.36
10.91
36.1
4.89
11.08
36.15
Standard deviation
0.31
0.55
1.13
0.27
0.63
1.1
C V (%)
5.87
5.06
3.13
5.61
5.73
3.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-10 in samples. No significant cross-reactivity or interference between Human IL-10 and analogues was observed.
