The Human IL-1 Beta ELISA Kit is a specialized assay designed for the precise quantification of interleukin-1 beta levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Interleukin-1 beta is a key pro-inflammatory cytokine that plays a crucial role in the immune response, inflammation, and various disease processes. Dysregulation of IL-1 beta has been implicated in conditions such as autoimmune diseases, inflammatory disorders, and certain types of cancer.
As such, measuring IL-1 beta levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapies.With its advanced technology and reliable performance, the Human IL-1 Beta ELISA Kit is an essential tool for researchers studying inflammatory pathways, immune responses, and disease mechanisms. This kit offers a convenient and efficient way to investigate IL-1 beta levels in human samples, making it a valuable addition to any laboratory's research arsenal.
Product Name:
Human IL-1 beta ELISA Kit
SKU:
HUES01423
Target:
Human IL-1 beta
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1β. You can calculate the concentration of Human IL-1β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
88-100
94-108
Average (%)
102
94
102
1:4
Range (%)
95-109
82-94
93-105
Average (%)
100
88
99
1:8
Range (%)
98-110
82-95
97-109
Average (%)
105
88
103
1:16
Range (%)
96-108
80-91
97-113
Average (%)
102
86
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.7
46.2
197.6
29.1
47.8
192.6
Standard deviation
1.4
2.6
9.5
1.7
2.8
6.0
C V (%)
5.05
5.63
4.81
5.84
5.86
3.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1β in samples. No significant cross-reactivity or interference between Human IL-1β and analogues was observed.
