The Human IL11 (Interleukin 11) CLIA Kit is a reliable and accurate assay designed for the quantitative detection of IL11 levels in human samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing high sensitivity and specificity for robust and reproducible results.IL11 is a cytokine known for its role in promoting hematopoiesis and inflammation, making it a valuable biomarker for studying various diseases and conditions. Its involvement in processes such as cell proliferation and differentiation underscores its importance in research related to cancer, autoimmune disorders, and inflammatory diseases.
With its easy-to-use format and precise results, the Human IL11 CLIA Kit is an essential tool for researchers looking to explore the function and implications of IL11 in different biological contexts. Get reliable data and valuable insights with this innovative assay kit from Assay Genie.
Product Name:
Human IL-11 CLIA Kit
SKU:
HUES01285
Target:
Human IL-11
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-11. You can calculate the concentration of Human IL-11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
101-121
93-106
Average (%)
102
110
99
1:4
Range (%)
91-104
90-104
89-102
Average (%)
98
97
96
1:8
Range (%)
97-112
84-96
100-115
Average (%)
103
90
105
1:16
Range (%)
93-106
99-115
99-115
Average (%)
99
107
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
93-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.73
116.68
312.97
44.21
109.03
284.76
Standard deviation
3.91
9.57
30.8
5.13
8.96
17.6
C V (%)
9.6
8.2
9.84
11.6
8.22
6.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-11 in samples. No significant cross-reactivity or interference between Human IL-11 and analogues was observed.
