The Human IL-12 CLIA Kit is a cutting-edge diagnostic tool designed for the precise and reliable measurement of interleukin-12 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.Interleukin-12 is a key cytokine that plays a critical role in regulating immune responses, particularly in the activation of T cells and natural killer cells. Dysregulation of IL-12 has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for understanding the underlying mechanisms of these conditions and developing targeted therapies.
Whether you are studying infectious diseases, autoimmune disorders, or immunotherapy, the Human IL-12 CLIA Kit from Assay Genie provides the precision and reliability you need to advance your research efforts. Unlock new insights into the complex interactions of the immune system with this state-of-the-art assay kit.
Product Name:
Human IL-12 CLIA Kit
SKU:
HUES00133
Target:
Human IL-12
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-12. You can calculate the concentration of Human IL-12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
90-108
92-106
Average (%)
108
98
97
1:4
Range (%)
98-112
96-111
91-103
Average (%)
105
104
96
1:8
Range (%)
89-100
94-107
87-98
Average (%)
95
100
93
1:16
Range (%)
86-97
98-116
87-102
Average (%)
92
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
98
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
100-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.75
101.46
316.46
43.49
101.51
318.17
Standard deviation
4.46
9.65
29.49
4.0
9.16
34.24
C V (%)
10.68
9.51
9.32
9.2
9.02
10.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-12 in samples. No significant cross-reactivity or interference between Human IL-12 and analogues was observed.
