The Human IL-13 CLIA Kit from AssayGenie is specifically designed for the quantification of Interleukin-13 (IL-13) levels in human samples such as serum, plasma, and cell culture supernatants. IL-13 is a key cytokine involved in inflammatory and immune responses, making it a valuable biomarker for studying various diseases such as asthma, allergy, and autoimmune disorders.
This CLIA kit offers high sensitivity and specificity, ensuring accurate and reliable results for your research needs. By measuring IL-13 levels, researchers can gain insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets. Trust AssayGenie's Human IL-13 CLIA Kit for your cytokine detection needs.
Product Name:
Human IL-13 CLIA Kit
SKU:
HUES00100
Target:
Human IL-13
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-13. You can calculate the concentration of Human IL-13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
83-99
99-116
Average (%)
98
90
106
1:4
Range (%)
94-109
88-101
96-113
Average (%)
100
94
103
1:8
Range (%)
85-98
87-99
89-101
Average (%)
92
93
96
1:16
Range (%)
91-104
87-100
95-107
Average (%)
98
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
105
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.98
72.2
209.32
22.14
68.68
216.92
Standard deviation
2.87
8.53
17.48
2.57
6.04
21.89
C V (%)
12.49
11.81
8.35
11.61
8.79
10.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-13 in samples. No significant cross-reactivity or interference between Human IL-13 and analogues was observed.
