The Human IL-15 (Interleukin-15) CLIA Kit is specifically designed for the quantitative detection of IL-15 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research purposes.IL-15 is a key cytokine involved in immune responses, particularly in the activation and proliferation of natural killer cells and T cells. Dysregulation of IL-15 has been implicated in various autoimmune disorders, inflammatory diseases, and cancer.
Therefore, measuring IL-15 levels can offer valuable insights into the immune system function and potentially aid in the development of novel therapies.Overall, the Human IL-15 CLIA Kit is a reliable tool for researchers studying immune responses, inflammation, and various diseases where IL-15 plays a critical role. Its precise and efficient performance make it a valuable addition to any laboratory conducting research on cytokines and immune system regulation.
Product Name:
Human IL-15 CLIA Kit
SKU:
HUES00178
Target:
Human IL-15
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-15. You can calculate the concentration of Human IL-15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
83-96
90-104
Average (%)
101
90
96
1:4
Range (%)
89-101
99-111
86-97
Average (%)
94
106
92
1:8
Range (%)
89-101
94-111
92-106
Average (%)
96
101
98
1:16
Range (%)
100-112
93-108
91-101
Average (%)
106
99
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
95-107
102
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.15
58.19
223.38
27.63
59.53
219.77
Standard deviation
2.5
5.51
23.61
2.95
4.44
19.82
C V (%)
9.94
9.47
10.57
10.68
7.46
9.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-15 in samples. No significant cross-reactivity or interference between Human IL-15 and analogues was observed.
