The Human IL-16 CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of IL-16 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it suitable for a variety of research applications.IL-16 is a cytokine that plays a key role in the regulation of immune responses and inflammatory processes. Abnormal levels of IL-16 have been associated with various diseases, including autoimmune disorders, inflammatory conditions, and certain types of cancer.
As such, IL-16 serves as an important biomarker for studying these diseases and potentially developing targeted therapies.Overall, the Human IL-16 CLIA Kit offers researchers a valuable tool for studying the role of IL-16 in health and disease, providing insights into potential diagnostic and therapeutic strategies.
Product Name:
Human IL-16 CLIA Kit
SKU:
HUES00182
Target:
Human IL-16
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-16. You can calculate the concentration of Human IL-16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
91-105
89-101
Average (%)
100
98
95
1:4
Range (%)
87-100
83-97
89-100
Average (%)
94
90
94
1:8
Range (%)
86-99
85-98
93-108
Average (%)
93
92
100
1:16
Range (%)
95-107
103-118
95-111
Average (%)
102
110
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
106
EDTA plasma (n=5)
97-115
105
Cell culture media (n=5)
98-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.53
118.16
319.07
40.58
124.15
346.73
Standard deviation
3.9
10.81
31.46
4.1
13.68
32.94
C V (%)
10.39
9.15
9.86
10.1
11.02
9.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-16 in samples. No significant cross-reactivity or interference between Human IL-16 and analogues was observed.
