The Human IL-18 CLIA Kit is specifically designed for the quantitative detection of Interleukin-18 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Interleukin-18 is a key cytokine involved in the inflammatory response, influencing immune cell activation and driving inflammatory processes in certain diseases. Its role in conditions such as autoimmune disorders, infectious diseases, and cancer makes it a valuable biomarker for understanding disease progression and developing targeted therapies.
With the Human IL-18 CLIA Kit, researchers can confidently measure IL-18 levels in biological samples, providing valuable insights into disease mechanisms and potential treatment strategies. Trust in this kit for precise and consistent results in your research endeavors.
Product Name:
Human IL-18 CLIA Kit
SKU:
HUES00188
Target:
Human IL-18
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-18. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-18. You can calculate the concentration of Human IL-18 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
101-115
86-99
Average (%)
104
107
92
1:4
Range (%)
97-110
86-99
87-100
Average (%)
104
93
95
1:8
Range (%)
94-110
98-112
85-99
Average (%)
101
105
92
1:16
Range (%)
86-100
101-116
97-110
Average (%)
92
106
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.99
54.46
235.63
27.39
54.86
213.0
Standard deviation
3.03
5.87
14.73
3.18
5.78
16.53
C V (%)
12.12
10.78
6.25
11.61
10.54
7.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-18 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-18 in samples. No significant cross-reactivity or interference between Human IL-18 and analogues was observed.
