The Human IL-19 CLIA Kit is specifically designed for the quantitative detection of IL-19 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.Interleukin-19, also known as IL-19, is a cytokine that plays a key role in regulating immune responses and inflammation. It is involved in various inflammatory processes and is thought to be a potential therapeutic target for inflammatory diseases such as asthma and psoriasis.
By measuring IL-19 levels, researchers can gain valuable insights into the immune response and inflammatory pathways, allowing for a better understanding of disease mechanisms and the development of targeted therapies. The Human IL-19 CLIA Kit provides a convenient and effective tool for studying the role of IL-19 in various physiological and pathological conditions.
Product Name:
Human IL-19 CLIA Kit
SKU:
HUES00189
Target:
Human IL-19
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-19. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-19, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-19. You can calculate the concentration of Human IL-19 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
92-108
101-118
Average (%)
90
99
108
1:4
Range (%)
102-116
89-100
88-102
Average (%)
109
94
93
1:8
Range (%)
88-104
95-108
100-114
Average (%)
95
101
108
1:16
Range (%)
96-111
94-106
88-100
Average (%)
101
100
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
107
EDTA plasma (n=5)
100-113
107
Cell culture media (n=5)
100-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.97
110.52
352.86
38.53
119.24
379.87
Standard deviation
3.57
11.59
24.49
4.83
12.46
25.11
C V (%)
9.16
10.49
6.94
12.54
10.45
6.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-19 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-19 in samples. No significant cross-reactivity or interference between Human IL-19 and analogues was observed.
