The Human IL-21 CLIA Kit is specifically designed for the precise measurement of interleukin-21 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Interleukin-21 is a key cytokine that plays a critical role in regulating immune responses and inflammation. It is involved in the development and function of immune cells, making it a valuable biomarker for studying immune-related diseases, autoimmune disorders, and infectious diseases.
With its advanced technology and reliable performance, the Human IL-21 CLIA Kit is the perfect tool for researchers looking to explore the role of interleukin-21 in health and disease, paving the way for potential therapeutic interventions and advancements in the field of immunology.
Product Name:
Human IL-21 CLIA Kit
SKU:
HUES01280
Target:
Human IL-21
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-21. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-21, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-21. You can calculate the concentration of Human IL-21 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
102-117
92-105
Average (%)
103
108
98
1:4
Range (%)
88-102
96-111
87-100
Average (%)
94
102
94
1:8
Range (%)
89-102
100-115
89-105
Average (%)
95
107
97
1:16
Range (%)
101-116
101-118
99-116
Average (%)
109
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.99
108.7
374.5
51.88
113.79
362.33
Standard deviation
4.82
8.37
27.6
4.31
11.48
23.3
C V (%)
8.93
7.7
7.37
8.31
10.09
6.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-21 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-21 in samples. No significant cross-reactivity or interference between Human IL-21 and analogues was observed.
