The Human IL-21 ELISA Kit is specifically designed for the precise detection of Interleukin-21 (IL-21) levels in human serum, plasma, and cell culture supernatants. With its advanced technology and high sensitivity, this kit ensures accurate and reproducible results, making it a valuable tool for a variety of research applications.IL-21 is a key cytokine that plays a critical role in immune regulation, inflammation, and the development of autoimmune diseases. By measuring IL-21 levels, researchers can gain insights into the immune response and potentially identify new therapeutic targets for various diseases.
This ELISA kit provides a simple and efficient method for quantifying IL-21 levels in biological samples, making it a valuable resource for scientists studying immunology, inflammation, and autoimmune disorders. With its reliable performance and ease of use, the Human IL-21 ELISA Kit is a valuable addition to any research laboratory.
Product Name:
Human IL-21 ELISA Kit
SKU:
HUES03314
Target:
Human IL-21
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-21. You can calculate the concentration of Human IL-21 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
90-104
91-106
Average (%)
100
96
97
1:4
Range (%)
87-103
84-98
81-97
Average (%)
95
90
88
1:8
Range (%)
91-101
83-96
84-95
Average (%)
96
89
89
1:16
Range (%)
86-101
83-97
85-98
Average (%)
93
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.19
568.57
1844.06
206.38
581.47
1961.3
Standard deviation
11.46
32.07
97.74
11.25
27.15
86.49
C V (%)
5.81
5.64
5.3
5.45
4.67
4.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-21 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-21 in samples. No significant cross-reactivity or interference between Human IL-21 and analogues was observed.
