The Human IL-24 CLIA Kit (HUES01266) offered by Assay Genie is a cutting-edge assay designed for the precise measurement of IL-24 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers highly accurate and reproducible results, making it an invaluable tool for various research applications. IL-24, also known as melanoma differentiation-associated gene 7 (mda-7), is a potent cytokine with anti-tumor properties that holds great promise for cancer therapy.
Its role in apoptosis, cell cycle arrest, and immune responses makes it a key target for studying cancer progression and developing novel treatment strategies. By detecting IL-24 levels, researchers can gain valuable insights into cancer biology and potentially identify new therapeutic avenues. Trust in the Human IL-24 CLIA Kit from Assay Genie to advance your research efforts and uncover the potential of IL-24 in cancer therapy and beyond. Visit www.assaygenie.com/human-il24-clia-kit-hues01266/ for more information.
Product Name:
Human IL-24 CLIA Kit
SKU:
HUES01266
Target:
Human IL-24
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-24. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-24, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-24. You can calculate the concentration of Human IL-24 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
90-102
98-114
Average (%)
98
95
105
1:4
Range (%)
94-105
86-98
84-96
Average (%)
99
91
91
1:8
Range (%)
86-97
88-103
99-114
Average (%)
92
94
104
1:16
Range (%)
98-112
103-116
85-97
Average (%)
106
108
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.26
58.64
204.1
28.72
54.72
221.22
Standard deviation
2.24
6.24
14.25
2.31
4.06
16.41
C V (%)
8.53
10.64
6.98
8.04
7.42
7.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-24 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-24 in samples. No significant cross-reactivity or interference between Human IL-24 and analogues was observed.
