The Human IL-25 ELISA Kit is specifically designed for the precise measurement of interleukin-25 (IL-25) levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for a variety of research applications.IL-25, also known as interleukin-17E, is a cytokine that plays a crucial role in regulating immune responses and inflammatory processes. It has been implicated in various conditions, including asthma, allergic diseases, and inflammatory bowel disease, making it a key target for study and potential therapeutic intervention.
By utilizing the Human IL-25 ELISA Kit, researchers can gain valuable insights into the role of IL-25 in disease pathogenesis and progression, ultimately leading to the development of novel treatment strategies. Trust in the accuracy and reliability of this kit to advance your research in the field of immunology and inflammation.
Product Name:
Human IL-25 ELISA Kit
SKU:
HUES02649
Target:
Human IL-25
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-25. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-25. You can calculate the concentration of Human IL-25 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
84-97
87-101
Average (%)
93
90
94
1:4
Range (%)
91-104
80-91
88-101
Average (%)
97
86
94
1:8
Range (%)
93-106
86-98
84-95
Average (%)
99
93
89
1:16
Range (%)
89-100
86-99
85-97
Average (%)
94
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.78
191.8
690.75
111.06
188.28
758.46
Standard deviation
5.93
8.17
28.74
6.33
8.51
39.74
C V (%)
5.55
4.26
4.16
5.7
4.52
5.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-25 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-25 in samples. No significant cross-reactivity or interference between Human IL-25 and analogues was observed.
