The Human IL-27 ELISA Kit is a powerful tool for the quantitative measurement of IL-27 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.IL-27 is a cytokine that plays a critical role in regulating the immune response and inflammation. It has been implicated in various autoimmune diseases, infectious diseases, and cancer.
By accurately measuring IL-27 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Human IL-27 ELISA Kit from Assay Genie is a valuable tool for researchers studying the role of IL-27 in health and disease, offering reliable results and easy-to-follow protocols for precise measurements.
Product Name:
Human IL-27 ELISA Kit
SKU:
HUES03213
Target:
Human IL-27
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-27. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-27. You can calculate the concentration of Human IL-27 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
85-97
97-112
Average (%)
99
91
103
1:4
Range (%)
93-108
87-97
84-96
Average (%)
99
92
89
1:8
Range (%)
90-102
83-95
90-102
Average (%)
96
90
95
1:16
Range (%)
88-103
81-94
84-95
Average (%)
94
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
93-106
98
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.62
231.08
749.46
96.41
247.43
809.0
Standard deviation
5.63
11.21
37.4
5.45
13.73
38.99
C V (%)
5.54
4.85
4.99
5.65
5.55
4.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-27 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-27 in samples. No significant cross-reactivity or interference between Human IL-27 and analogues was observed.
