The Human IL-3 CLIA Kit is specifically designed for the quantitative measurement of Interleukin-3 (IL-3) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.IL-3 is a key cytokine that regulates the growth, differentiation, and survival of various immune cells, including mast cells, basophils, and eosinophils. Abnormal levels of IL-3 have been associated with various inflammatory, allergic, and autoimmune diseases, making it a valuable biomarker for studying these conditions.
By utilizing the Human IL-3 CLIA Kit, researchers can gain valuable insights into the role of IL-3 in disease pathogenesis, potentially leading to the development of novel diagnostic tools and therapeutic interventions. With its superior performance and ease of use, this kit is an essential tool for investigating the complex mechanisms of immune regulation and inflammatory responses.
Product Name:
Human IL-3 CLIA Kit
SKU:
HUES00096
Target:
Human IL-3
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-3. You can calculate the concentration of Human IL-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
99-109
100-115
Average (%)
94
104
109
1:4
Range (%)
87-99
94-105
100-111
Average (%)
94
99
105
1:8
Range (%)
99-117
87-101
88-98
Average (%)
107
93
93
1:16
Range (%)
86-100
98-111
92-109
Average (%)
93
104
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
106
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.79
79.9
222.34
24.0
72.97
209.45
Standard deviation
2.29
6.26
14.45
2.69
8.36
16.38
C V (%)
10.05
7.83
6.5
11.21
11.46
7.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-3 in samples. No significant cross-reactivity or interference between Human IL-3 and analogues was observed.
