The Human IL-3 ELISA Kit is a powerful tool for measuring interleukin-3 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing precise and consistent results for various research applications.Interleukin-3 is a key cytokine that plays a vital role in the regulation of immune responses, hematopoiesis, and inflammation. Abnormal levels of IL-3 have been associated with various diseases, including cancer, autoimmune disorders, and allergic reactions, highlighting its importance as a biomarker for disease diagnosis and treatment monitoring.
With its reliable performance and broad utility, the Human IL-3 ELISA Kit is an indispensable tool for researchers exploring the complex role of interleukin-3 in health and disease. Get accurate and insightful data to advance your research with this cutting-edge kit from Assay Genie.
Product Name:
Human IL-3 ELISA Kit
SKU:
HUES01386
Target:
Human IL-3
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-3. You can calculate the concentration of Human IL-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-111
92-102
94-108
Average (%)
101
97
100
1:4
Range (%)
89-102
84-96
87-101
Average (%)
94
88
92
1:8
Range (%)
86-100
83-95
86-96
Average (%)
93
90
91
1:16
Range (%)
88-99
79-93
87-101
Average (%)
93
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.04
159.61
356.96
47.69
156.29
343.47
Standard deviation
2.63
8.16
13.24
3.31
7.64
13.19
C V (%)
5.59
5.11
3.71
6.94
4.89
3.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-3 in samples. No significant cross-reactivity or interference between Human IL-3 and analogues was observed.
