The Human IL-31 ELISA Kit is specifically designed for the precise measurement of Interleukin-31 (IL-31) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.IL-31 is a key cytokine that plays a critical role in inflammatory responses and immune regulation. Elevated levels of IL-31 have been linked to various inflammatory skin conditions, such as atopic dermatitis and psoriasis, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With its high performance and reliability, the Human IL-31 ELISA Kit is an essential tool for researchers and clinicians interested in investigating the role of IL-31 in inflammatory disorders and developing targeted treatments.
Product Name:
Human IL-31 ELISA kit
SKU:
HUES03458
Target:
Human IL-31 ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-31. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-31 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-31, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-31. You can calculate the concentration of Human IL-31 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
85-99
97-111
Average (%)
102
90
104
1:4
Range (%)
86-100
84-99
82-96
Average (%)
93
91
88
1:8
Range (%)
93-104
85-96
83-97
Average (%)
98
90
90
1:16
Range (%)
93-109
79-93
84-97
Average (%)
99
85
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.25
78.19
180.25
22.72
70.66
191.57
Standard deviation
1.4
4.23
5.91
1.45
3.07
7.43
C V (%)
5.77
5.41
3.28
6.38
4.34
3.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-31 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-31 in samples. No significant cross-reactivity or interference between Human IL-31 and analogues was observed.
