The Human IL-33 CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of IL-33 levels in human serum, plasma, and cell culture supernatants. This kit provides researchers with the tools needed to study the role of IL-33 in various biological processes and disease states. IL-33 is a key cytokine involved in inflammation and immune responses, playing a critical role in conditions such as asthma, autoimmune diseases, and allergic reactions. By accurately measuring IL-33 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.
The Human IL-33 CLIA Kit offers high specificity and reproducibility, ensuring that researchers can confidently rely on the results obtained. Whether studying basic biology or exploring potential treatment options, this kit is an essential tool for advancing research in immunology and related fields.
Product Name:
Human IL-33 CLIA Kit
SKU:
HUES01249
Target:
Human IL-33
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-33. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-33 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-33, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-33. You can calculate the concentration of Human IL-33 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
85-98
99-115
Average (%)
95
91
105
1:4
Range (%)
102-113
89-105
90-103
Average (%)
107
96
97
1:8
Range (%)
86-101
89-103
96-112
Average (%)
93
95
103
1:16
Range (%)
92-107
93-108
92-106
Average (%)
99
99
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
92-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.16
68.85
187.61
26.17
64.4
182.87
Standard deviation
2.98
5.0
14.01
3.13
5.57
19.8
C V (%)
12.33
7.26
7.47
11.96
8.65
10.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-33 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-33 in samples. No significant cross-reactivity or interference between Human IL-33 and analogues was observed.
