The Human IL-34 ELISA Kit is specifically designed for the quantitative measurement of interleukin 34 (IL-34) levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.IL-34 is a cytokine that plays a vital role in regulating the immune response and inflammation in various physiological and pathological conditions. Its dysregulation has been implicated in autoimmune diseases, inflammatory disorders, and cancer.
Therefore, measuring IL-34 levels is essential for understanding disease pathogenesis and developing targeted therapeutic strategies.With its reliable performance and ease of use, the Human IL-34 ELISA Kit is an invaluable tool for researchers studying the role of IL-34 in health and disease. Get yours today and unlock new insights into the complex mechanisms underlying immune regulation and inflammation.
Product Name:
Human IL-34 ELISA Kit
SKU:
HUES02650
Target:
Human IL-34
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-34. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-34 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-34, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-34. You can calculate the concentration of Human IL-34 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
94-104
95-111
Average (%)
104
99
102
1:4
Range (%)
86-102
81-94
81-95
Average (%)
94
86
88
1:8
Range (%)
87-101
86-97
84-97
Average (%)
94
91
91
1:16
Range (%)
92-104
80-93
88-100
Average (%)
97
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
292
719.71
2331.63
295.64
706.23
2493.5
Standard deviation
15.07
38.22
90.47
15.46
39.62
112.71
C V (%)
5.16
5.31
3.88
5.23
5.61
4.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-34 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-34 in samples. No significant cross-reactivity or interference between Human IL-34 and analogues was observed.
