The Human IL4 CLIA Kit is a cutting-edge assay designed for the precise measurement of IL4 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it a valuable tool for various research purposes.Interleukin-4 (IL4) is a key cytokine that plays a critical role in modulating immune responses and inflammatory processes. Dysregulation of IL4 levels has been implicated in various diseases and conditions, including asthma, allergies, and autoimmune disorders.
As a result, IL4 serves as an important biomarker for studying these conditions and developing potential therapeutic interventions.For researchers seeking to explore the role of IL4 in health and disease, the Human IL4 CLIA Kit offers a reliable and efficient solution for quantifying IL4 levels with precision and accuracy. Elevate your research with this state-of-the-art assay kit from AssayGenie.
Product Name:
Human IL-4 CLIA Kit
SKU:
HUES00097
Target:
Human IL-4
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-4. You can calculate the concentration of Human IL-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
87-102
98-113
Average (%)
99
93
104
1:4
Range (%)
96-109
93-108
85-96
Average (%)
101
101
90
1:8
Range (%)
86-102
86-101
90-107
Average (%)
93
92
98
1:16
Range (%)
93-108
96-109
86-99
Average (%)
99
102
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
87-102
95
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.6
103.91
309.08
43.94
109.99
299.01
Standard deviation
5.15
9.58
28.59
4.53
10.74
23.89
C V (%)
12.38
9.22
9.25
10.31
9.76
7.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
